The queuing theory is applied to calculating the intercept efficiency of the mixed multiplayer disposition. Taking a three multiplayer disposition for example, based on some hypothesis and using the queuing theory, a model of intercept efficiency of a three multiplayer disposition is built. First, the intercept efficiency of each layer is calculated, based on which the total efficiency is got, and the penetration probability of the cruise missile is calculated. Finally, an example is given to verify the practicability and feasibility of the model. This method can be used to calculate the intercept efficiency when disposition schemes are given, it can also be used to get the disposition schemes to meet an intercepting efficiency. This can provide a reference basis for making and implementing the disposition schemes, and simultaneously provide some relative information for the commander in decision making.