The software simulation of aviation wireless navigation system is the basis of the simulation of flight check, and also is an important component of comprehensive simulation system of air force communication system. The characteristics of aviation wireless navigation system and of the simulation of it are analyzed, and simultaneously the characteristics of Matlab/Simulink in modeling simulation are analyzed. At the same time, its extensibility and reusability representing in the compatibility with HLA are also analyzed. An initiative research of the simulation of aviation wireless navigation system is made by using Matlab/Simulink. The Simulink models of the subsystems of ground course beacon, antenna distribution network, spatial signal synthesis and receiver onboard subsystems of Instrument Landing System are built and the correct signal wave-form produced by the simulation system is obtained. A resolution scheme about joining the Simulink model of Instrument Landing System into the comprehensive simulation system federation of air force communication system is put forward.